Problem: Dilutehumanepithelialcolorectaladenocarcinoma(Caco-2)cellsincellmediumandplace200-400μlofthecellsolutionintotheupper(apical)chamberofthetranswellinsert,atadensityof1.5x105cells/cm2.Fillthelower(basolateral)chamberwith700-900μlofcellmedium.
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Dilute human epithelial colorectal adenocarcinoma (Caco-2) cells in cell medium and place 200-400 μl of the cell solution into the upper (apical) chamber of the transwell insert, at a density of 1.5 x 105 cells/cm2. Fill the lower (basolateral) chamber with 700 - 900 μl of cell medium.


Problem: 11Truthshallspringoutoftheearth;andrighteousnessshalllookdownfromheaven.
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11 Truth shall spring out of the earth; and righteousness shall look down from heaven.


Problem: AfterthisvisityouwillenjoylunchbeforeyouwillgotoTunael-Gebel,thenecropolisoftheoldcityofKhmun,alsoknownasHermopolisMagna.
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After this visit you will enjoy lunch before you will go to Tuna el-Gebel, the necropolis of the old city of Khmun, also known as Hermopolis Magna.


Problem: OfcourseitisalmostcertainthatRecordehadavalidcaseagainstPembrokebuttherewasnowaythatsomeonesoclosetoMaryandPhilipwouldallowaminorcivilservantlikeRecordetogetthebetterofhim.
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Of course it is almost certain that Recorde had a valid case against Pembroke but there was no way that someone so close to Mary and Philip would allow a minor civil servant like Recorde to get the better of him.